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Biological cleaning of stone by means of desulphurazing bacteria

Provider: Univerzita Pardubice
Programme: Studentská grantová soutěž
Implementation period: 01.02.22 - 31.12.22
Workplace: Fakulta restaurování - FR
Investigator: Zítková Petra
Description:
Gypsum crusts damage the stone artworks not only from an aesthetic, but especially from a technological point of view, therefore their removal is often a common issue of restoration intervention. Black crust is a deteriorated surface layer of stone artworks spontaneously formed on calcareous substrate. The project will be focused on the possibilities of black crust removal from stone surface using bacterial cleaning system. Main aims of the research include investigation of effectiveness of sulphate-reducing bacteria used for the removal of black crusts, the assessment of their invasiveness on the artwork surfaces and any considerations and improvements in the field of practical applications. The cleaning system is based on Desulfovibrio vulgaris, the sulfate-reducing bacteria. The cleaning and application techniques will be performed on stone model samples (limestone or marble) and evaluated after the treatment by the means such as visual observation, photographic both in visible light and UV luminescence, documentation, optical microscopy and scanning electron microscopy